Mouse anti Eotaxin

Catalog numberX1900M
NameMouse anti Eotaxin
Size500 μg
Price474.00 EUR
Extended details
CategoryPrimary Antibodies
Long descriptionEotaxin is a potent eosinophil chemoattractant which was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig eotaxin to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human eotaxin have recently been isolated. Mouse eotaxin cDNA encodes a 97 amino acid protein from which the amino-terminal 23 amino acid are cleaved to generate the 74 amino acid mature mouse eotaxin. Mature mouse eotaxin is approximately 60% identical to mature human and guinea pig eotaxin. In addition, mouse eotaxin also shows high amino acid sequence identity to members of the MCP family. Mouse eotaxin is chemotactic for eosinophils, but not mononuclear cells or neutrophils. Eotaxin mRNA is expressed in a variety of tissues. The expression of eotaxin mRNA is induced in cultured endothelial cells in response to IFN- . In addition, eotaxin mRNA is also induced in response to the transplantation of IL-4-secreting tumor cells. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human eotaxin receptor.
Antibody come fromHybridoma produced by the fusion of splenocytes from rat immunized with mouse recombinant IL-2 protein and P3X63-AG8.653 myeloma cells.
Other descriptionProvided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
CloneA132D 48F9
Antigen-antibody binding interactionMouse anti Eotaxin Antibody
Antibody is raised inMouse
Antibody's reacts withHuman
Antibody's reacts with these speciesThis antibody doesn't cross react with other species
Antibody's specificityNo Data Available
ApplicationELISA (capture antibody)
Antibody's suited forAntibody can be used for ELISA as a coating antibody (1-5µg/ml, 100 µl/well). and immunoprecipitation. Optimal concentration should be evaluated by serial dilutions._x000B_Suggested Working Dilutions: Immediately prior to use as a coating antibody in ELISA, dilute this preparation to a final concentration of 1-5 µg/mL, and coat each well of a microtiter plate with 100 µL. A general ELISA procedure is available upon request. Alternatively, the antibody may be diluted in buffered solution with carrier protein, such as phosphate buffered saline supplemented with 0.1-1.0% bovine serum albumin. The optimal antibody concentration should be determined for each specific application.
Relevant referencesAbrams, J.S., M.G. Roncarolo, H. Yssel, U. Andersson, G.J. Gleich, and J.E. Silver (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunological Review 127:5-24.
Protein numbersee ncbi
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus