Mouse CCL24/Eotaxin-2 PicoKine ELISA Kit

Information
Catalog numberEK0576
NameMouse CCL24/Eotaxin-2 PicoKine ELISA Kit
Size1x96 well plate
Price556.70 EUR
Supplierboster
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Extended details
ELISA's applicationFor quantitative measurement of mouse CCL24 in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
Gene testedmurine CCL24
ELISA standard curveConcentration(pg/ml) + 0 + 15.6 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 O.D. + 0.081 + 0.163 + 0.251 + 0.405 + 0.696 + 1.219 + 1.891 + 2.345
ELISA kit detection limits15.6pg/ml to 1000pg/ml
ELISA's sensitivity<10pg/ml
Target of this ELISANatural and recombinant mouse CCL24
Cross reactionthe ELISA does not cross react with any other proteins except from closely related species
ELISA's precision parametersintra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);125;257;526;130;249;518 ELISA standard deviations 6;10.79;33.14;10.14;15.94;42.99 CV(%);4.8;4.2;6.3;7.8;6.4;8.3
ELISA kit descriptionGENTAUR's mouse CCL24 ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for CCL24 was used as capure antibodies on the ELISA test plate. The internal standards (E.coli, V27-V119) and your samples are measured with a biotin conjugated polyclonal from goat specific for CCL24 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse CCL24 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
Detector and capure antibodiesA rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.
The ELISA containsCatalog number;Description;Quantity -;96-well plate precoated with anti- mouse CCL24 antibodies;1 ST0000-10;Lyophilized recombinant mouse CCL24 standard;10ng/tubex2 AR1107;Biotinylated anti- mouse CCL24 antibodies;130ul(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex(ABC);130ul(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Anti-coagulationheparin or EDTA
How the elisa worksSample the amount of 100ul/well of the standard solution at 1000 pg per ml, 500, 25, 125, 62.5, 31.25, 15.6 pg/ml mouse CCL24 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, serum or plasma(heparin, EDTA) to the ELISA plate. Keep in mind that each mouse CCL24 standard solution and each sample be measured in duplicate.
Minutes of TMB incubation25-30
More gene infoChemokine(C-C motif) ligand 24(CCL24) also known as myeloid progenitor inhibitory factor 2(MPIF-2) or eosinophil chemotactic protein 2(eotaxin-2) is a protein that in humans is encoded by the CCL24 gene.1 By use of PCR analysis of somatic cell hybrid DNAs, radiation hybrid mapping, and a chromosome 7-specific YAC library, mapped the SCYA24 gene to chromosome 7q11.23.2 CCL24 is a small cytokine belonging to the CC chemokine family. CCL24 interacts with chemokine receptor CCR3 to induce chemotaxis in eosinophils.3 This chemokine is also strongly chemotactic for resting T lymphocytes and slightly chemotactic for neutrophils.
AntigenE.coli, V27-V119
Keep atStore at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
UniprotC-C motif chemokine 24
Gene namechemokine (C-C motif) ligand 24
AlternativeC C motif chemokine 24;CCL24;Chemokine (C C motif) ligand 24;Chemokine CC Motif Ligand 2;CK beta 6;Ckb 6;Ckb6;Eosinophil chemotactic protein 2;Eotaxin 2 like protein;MPIF 2;MPIF2;Myeloid progenitor inhibitory factor 2;OTTHUMP00000210225;SCYA24;Small inducible cytokine A24;Small inducible cytokine subfamily A (Cys Cys) member 24
Gen protein identityQ9JKC0
Entrez nr.56221
PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus