Human CCL24/Eotaxin-2 PicoKine ELISA Kit

Information
Catalog numberEK0331
NameHuman CCL24/Eotaxin-2 PicoKine ELISA Kit
Size1x96 well plate
Price509.90 EUR
Supplierboster
Order
Extended details
ELISA's applicationFor quantitative measurement of human CCL24 in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
Gene testedHuman CCL24
ELISA standard curveConcentration(pg/ml) + 0 + 78 + 156 + 313 + 625 + 1250 + 2500 + 5000 O.D. + 0.040 + 0.222 + 0.377 + 0.684 + 1.159 + 1.741 + 1.979 + 2.121
ELISA kit detection limits78pg/ml to 5000pg/ml
ELISA's sensitivity<2pg/ml
Target of this ELISANatural and recombinant human CCL24
Cross reactionthe ELISA does not cross react with any other proteins except from closely related species
ELISA's precision parametersintra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);525;1357;2556;630;1749;3018 ELISA standard deviations 40.95;97.7;158.5;55.44;129.4;268.6 CV(%);7.8;7.2;6.2;8.8;7.4;8.9
ELISA kit descriptionGENTAUR's human CCL24 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for CCL24 was used as capure antibodies on the ELISA test plate. The internal standards (E.coli, V27-C119) and your samples are measured with a biotin conjugated polyclonal from goat specific for CCL24 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human CCL24 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
Detector and capure antibodiesa monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
The ELISA containsCatalog number;Description;Quantity -;96-well plate precoated with anti- human CCL24 antibodies;1 ST0000-10;Lyophilized recombinant human CCL24 standard;10ng/tube×2 AR1107;Biotinylated anti- human CCL24 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Anti-coagulationheparin or EDTA
How the elisa worksAliquot 0.1ml per well of 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 313pg/ml, 156pg/ml, 78pg/ml human CCL24 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum or plasma(heparin, EDTA) to the ELISA plate. Keep in mind that each human CCL24 standard solution and each sample be measured in duplicate.
Minutes of TMB incubation15-20
More gene infoChemokine(C-C motif) ligand 24(CCL24) also known as myeloid progenitor inhibitory factor 2(MPIF-2) or eosinophil chemotactic protein 2(eotaxin-2) is a protein that in humans is encoded by the CCL24 gene. By use of PCR analysis of somatic cell hybrid DNAs, radiation hybrid mapping, and a chromosome 7-specific YAC library, mapped the SCYA24 gene to chromosome 7q11.23. CCL24 is a small cytokine belonging to the CC chemokine family. CCL24 interacts with chemokine receptor CCR3 to induce chemotaxis in eosinophils.3 This chemokine is also strongly chemotactic for resting T lymphocytes and slightly chemotactic for neutrophils.
AntigenE.coli, V27-C119
Keep atStore at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
UniprotC-C motif chemokine 24
Gene namechemokine (C-C motif) ligand 24
AlternativeC C motif chemokine 24;CCL24;Chemokine (C C motif) ligand 24;Chemokine CC Motif Ligand 2;CK beta 6;Ckb 6;Ckb6;Eosinophil chemotactic protein 2;Eotaxin 2 like protein;MPIF 2;MPIF2;Myeloid progenitor inhibitory factor 2;OTTHUMP00000210225;SCYA24;Small inducible cytokine A24;Small inducible cytokine subfamily A (Cys Cys) member 24
Gen protein identityO00175
Entrez nr.6369
PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED