Mouse Eotaxin PicoKine ELISA Kit

Information
Catalog numberEK0330
NameMouse Eotaxin PicoKine ELISA Kit
Size1x96 well plate
Price493.00 EUR
Supplierboster
Order
Extended details
ELISA's applicationFor quantitative measurement of mouse Eotaxin in cell culture supernatant, serum and plasma citrate, EDTA .
Gene testedmurine Eotaxin
ELISA standard curveConcentration(pg/ml) + 0 + 15.6 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 O.D. + 0.064 + 0.193 + 0.331 + 0.525 + 0.990 + 1.375 + 1.678 + 1.952
ELISA kit detection limits15.6pg/ml to 1000pg/ml
ELISA's sensitivity<1pg/ml
Target of this ELISANatural and recombinant mouse Eotaxin
Cross reactionthe ELISA does not cross react with any other proteins except from closely related species
ELISA's precision parametersintra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);81;201;475;98;189;458 ELISA standard deviations 5.59;9.05;33.73;8.7;13.99;38.01 CV(%);6.9;4.5;7.1;8.9;7.4;8.3
ELISA kit descriptionGENTAUR's mouse Eotaxin ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for Eotaxin was used as capure antibodies on the ELISA test plate. The internal standards (E.coli, H24-P97) and your samples are measured with a biotin conjugated polyclonal from goat specific for Eotaxin will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse Eotaxin in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
Detector and capure antibodiesA rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.
The ELISA containsCatalog number;Description;Quantity -;96-well plate precoated with anti- mouse Eotaxin antibodies;1 ST0000-10;Lyophilized recombinant mouse Eotaxin standard;10ng/tube×2 AR1107;Biotinylated anti- mouse Eotaxin antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Anti-coagulationcitrate or EDTA
How the elisa worksSample the amount of 100ul/well of the standard solution at 1000 pg per ml, 500, 25, 125, 62.5, 31.25, 15.6 pg/ml mouse Eotaxin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, serum or plasma(citrate, EDTA) to the ELISA plate. Keep in mind that each mouse Eotaxin standard solution and each sample be measured in duplicate.
Minutes of TMB incubation15-20
More gene infoEotaxin, also known as CCL11, is a potent inducer of eosinophil chemotaxis and is considered as a selective ligand of the CC chemokine receptor 3(CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes. Eotaxin is assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. The gene maps to chromosome 17 and is expressed constitutively at high levels in small intestine and colon, and at lower levels in various other tissues. The deduced mature protein sequence is 66% identical to human monocyte chemoattractant protein-1, and 60% identical to guinea pig eotaxin.
AntigenE.coli, H24-P97
Keep atStore at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
UniprotEotaxin
Gene namechemokine (C-C motif) ligand 11
AlternativeC-C motif chemokine 11;CCL 11;CCL11;CCL11_HUMAN;Chemokine (C-C motif) ligand 11;Chemokine C C motif ligand 11;Chemokine CC Motif Ligand 11;Chemokine ligand 11;Eosinophil Chemotactic P;Eosinophil chemotactic protein;Eotaxin;Eotaxin 1;MGC22554;SCYA 11;SCYA11;Small inducible cytokine A11;Small inducible cytokine subfamily A (Cys Cys) member 11;Small inducible cytokine subfamily A member 11;Small-inducible cytokine A11
Gen protein identityP48298
Entrez nr.20292
PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin nameMus musculus