Information
| Catalog number |
EK0329 |
| Name |
Human Eotaxin PicoKine ELISA Kit |
| Size |
1x96 well plate |
| Price |
493.00 EUR |
| Supplier |
boster
|
|
Order
|
| ELISA's application |
For quantitative measurement of human Eotaxin in cell culture supernatant, serum and plasma citrate,EDTA . |
| Gene tested |
Human Eotaxin |
| ELISA standard curve |
Concentration(pg/ml) + 0 + 15.6 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 O.D. + 0.030 + 0.091 + 0.167 + 0.301 + 0.568 + 1.134 + 1.877 + 2.273 |
| ELISA kit detection limits |
15.6pg/ml to 1000pg/ml |
| ELISA's sensitivity |
< 2pg/ml |
| Target of this ELISA |
Natural and recombinant human Eotaxin |
| Cross reaction |
the ELISA does not cross react with any other proteins except from closely related species |
| ELISA's precision parameters |
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);83.4;205;468;84.5;202;485 ELISA standard deviations 3.5;12.5;19.2;8.2;19.8;44.1 CV(%);4.2;6.1;4.1;9.7;9.8;9.1 |
| ELISA kit description |
GENTAUR's human Eotaxin ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for Eotaxin was used as capure antibodies on the ELISA test plate. The internal standards (E.coli, G24-P97) and your samples are measured with a biotin conjugated polyclonal from goat specific for Eotaxin will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human Eotaxin in your sample the higher the ELISA signal will be compared to this elisa's standard curve. |
| Detector and capure antibodies |
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat |
| The ELISA contains |
Catalog number;Description;Quantity -;96-well plate precoated with anti- human Eotaxin antibodies;1 ST0000-10;Lyophilized recombinant human Eotaxin standard;10ng/tube×2 AR1107;Biotinylated anti- human Eotaxin antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4 |
| Anti-coagulation |
citrate or EDTA |
| How the elisa works |
Sample the amount of 100ul/well of the standard solution at 1000 pg per ml, 500, 25, 125, 62.5, 31.25, 15.6 pg/ml human Eotaxin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum or plasma(citrate,EDTA) to the ELISA plate. Keep in mind that each human Eotaxin standard solution and each sample be measured in duplicate. |
| Minutes of TMB incubation |
20-25 |
| More gene info |
Eotaxin, also known as CCL11, is a potent inducer of eosinophil chemotaxis and is considered as a selective ligand of the CC chemokine receptor 3(CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes. Eotaxin is assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. The gene maps to chromosome 17 and is expressed constitutively at high levels in small intestine and colon, and at lower levels in various other tissues. The deduced mature protein sequence is 66% identical to human monocyte chemoattractant protein-1, and 60% identical to guinea pig eotaxin. Recombinant human eotaxin produced in insect cells induces a calcium flux response in normal human eosinophils, but not in neutrophils or monocytes. The human eotaxin gene is cloned and found to be 61.8 and 63.2% identical at the amino acid level to guinea pig and mouse eotaxin. |
| Antigen |
E.coli, G24-P97 |
| Keep at |
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Uniprot |
Eotaxin |
| Gene name |
chemokine (C-C motif) ligand 11 |
| Alternative |
C-C motif chemokine 11;CCL 11;CCL11;CCL11_HUMAN;Chemokine (C-C motif) ligand 11;Chemokine C C motif ligand 11;Chemokine CC Motif Ligand 11;Chemokine ligand 11;Eosinophil Chemotactic P;Eosinophil chemotactic protein;Eotaxin;Eotaxin 1;MGC22554;SCYA 11;SCYA11;Small inducible cytokine A11;Small inducible cytokine subfamily A (Cys Cys) member 11;Small inducible cytokine subfamily A member 11;Small-inducible cytokine A11 |
| Gen protein identity |
P51671 |
| Entrez nr. |
6356 |
| Properties |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. |
| Test |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |