Human ECF/CCL11 (Eosinophil Chemotactic Factor) ELISA Kit

Information
Catalog numberEKE60024
NameHuman ECF/CCL11 (Eosinophil Chemotactic Factor) ELISA Kit
Size96 Tests
Price759.00 EUR
SupplierBiomatik
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Extended details
Estimated Turnaround Time6-11 business days
Target NameNA
Target's alterntive nameECF/CCL11
Species ReactivityHuman
Sample TypeSerum, Plasma, Biological Fluids
Detection Range15.63-1000 pg/mL
Sensitivity9.38 pg/mL
PrecisionNA
Detection MethodColorimetric
Assay Time4.5 hours
ApplicationFor research use only. Not for diagnostic procedures.
Assay TypeSandwich
Shipping ConditionsIce packs
Storage TemperatureShort term: 4°C; Long term: see manual.
Precaution of UseThe Stop Solution is acidic. Do not allow to contact skin or eyes.
Shelf lifeUse Human ECF/CCL11 (Eosinophil Chemotactic Factor) ELISA Kit before 6 months
SpecificityNA
Cross ActivityNo significant cross-reactivity or interference was observed.
Test PrincipleThis ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ECF/CCL11 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ECF/CCL11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ECF/CCL11, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ECF/CCL11. The concentration of Human ECF/CCL11 in samples can be calculated by comparing the OD of the samples to the standard curve.
StabilityNA
Quality SystemsThe kit is manufactured at ISO 9001 certified facilities.
Research AreaCancer, Immunology
PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
DescriptionAplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.