Information
| Catalog number |
EK0576 |
| Name |
Mouse CCL24/Eotaxin-2 PicoKine ELISA Kit |
| Size |
1x96 well plate |
| Price |
556.70 EUR |
| Supplier |
boster
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Order
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| ELISA's application |
For quantitative measurement of mouse CCL24 in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA . |
| Gene tested |
murine CCL24 |
| ELISA standard curve |
Concentration(pg/ml) + 0 + 15.6 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 O.D. + 0.081 + 0.163 + 0.251 + 0.405 + 0.696 + 1.219 + 1.891 + 2.345 |
| ELISA kit detection limits |
15.6pg/ml to 1000pg/ml |
| ELISA's sensitivity |
< 10pg/ml |
| Target of this ELISA |
Natural and recombinant mouse CCL24 |
| Cross reaction |
the ELISA does not cross react with any other proteins except from closely related species |
| ELISA's precision parameters |
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);125;257;526;130;249;518 ELISA standard deviations 6;10.79;33.14;10.14;15.94;42.99 CV(%);4.8;4.2;6.3;7.8;6.4;8.3 |
| ELISA kit description |
GENTAUR's mouse CCL24 ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for CCL24 was used as capure antibodies on the ELISA test plate. The internal standards (E.coli, V27-V119) and your samples are measured with a biotin conjugated polyclonal from goat specific for CCL24 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse CCL24 in your sample the higher the ELISA signal will be compared to this elisa's standard curve. |
| Detector and capure antibodies |
A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies. |
| The ELISA contains |
Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse CCL24 antibodies;1 ST0000-10;Lyophilized recombinant mouse CCL24 standard;10ng/tubex2 AR1107;Biotinylated anti- mouse CCL24 antibodies;130ul(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex(ABC);130ul(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4 |
| Anti-coagulation |
heparin or EDTA |
| How the elisa works |
Sample the amount of 100ul/well of the standard solution at 1000 pg per ml, 500, 25, 125, 62.5, 31.25, 15.6 pg/ml mouse CCL24 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, serum or plasma(heparin, EDTA) to the ELISA plate. Keep in mind that each mouse CCL24 standard solution and each sample be measured in duplicate. |
| Minutes of TMB incubation |
25-30 |
| More gene info |
Chemokine(C-C motif) ligand 24(CCL24) also known as myeloid progenitor inhibitory factor 2(MPIF-2) or eosinophil chemotactic protein 2(eotaxin-2) is a protein that in humans is encoded by the CCL24 gene.1 By use of PCR analysis of somatic cell hybrid DNAs, radiation hybrid mapping, and a chromosome 7-specific YAC library, mapped the SCYA24 gene to chromosome 7q11.23.2 CCL24 is a small cytokine belonging to the CC chemokine family. CCL24 interacts with chemokine receptor CCR3 to induce chemotaxis in eosinophils.3 This chemokine is also strongly chemotactic for resting T lymphocytes and slightly chemotactic for neutrophils. |
| Antigen |
E.coli, V27-V119 |
| Keep at |
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Uniprot |
C-C motif chemokine 24 |
| Gene name |
chemokine (C-C motif) ligand 24 |
| Alternative |
C C motif chemokine 24;CCL24;Chemokine (C C motif) ligand 24;Chemokine CC Motif Ligand 2;CK beta 6;Ckb 6;Ckb6;Eosinophil chemotactic protein 2;Eotaxin 2 like protein;MPIF 2;MPIF2;Myeloid progenitor inhibitory factor 2;OTTHUMP00000210225;SCYA24;Small inducible cytokine A24;Small inducible cytokine subfamily A (Cys Cys) member 24 |
| Gen protein identity |
Q9JKC0 |
| Entrez nr. |
56221 |
| Properties |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain. |
| Latin name |
Mus musculus |